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Figure 4 Quantitative Analysis of Perfusion Measured With CPS Ultrasound and MRI in Rabbit Hind Limbs 6 Days After AdVEGF GT

(A) Blood volume in the muscle is increased 41- to 51-fold after AdVEGF (n = 9) gene transfer (GT) as measured by BV-MRI and CPS ultrasound, respectively. (B) Perfusion is increased 20- to 24-fold after AdVEGF treatment, depending on the method used. (C) Arrival time is shortened in AdVEGF-injected muscles, reflecting a decrease in peripheral resistance owing to vessel enlargement. In contrast, MTT is increased after AdVEGF GT. (D) Capillary mean size is 29-fold larger in AdVEGF-treated muscles than in AdLacZ (n = 10) muscles. (E) Capillary density does not change after AdVEGF GT in 6 days. (F) Of AdVEGF-treated muscles, 9.2% are covered by capillary lumens, whereas normal capillary total area in AdLacZ muscles is 0.3%. *p < 0.05, **p < 0.01, and ***p < 0.001 versus AdLacZ. Analyzed by the Kruskal-Wallis test, followed by Mann-Whitney U test. Abbreviations as in Figures 1 and 2.