Click on image to view larger version.

Figure 4 Pathologic Characterization APA Localization

The remote zone (A) demonstrates no APA uptake in interstitium (*) or cardiomyocytes (arrows). The cells of nonmyocytic origin demonstrate fluorescent APA uptake in the infarct zone (B), with an enlarged image of cellular uptake (inset). These cells in the infarct region (D, green) also stained positively for smooth muscle actin (SMA) (E, red), showing colocalization (F, yellow), indicating AT receptors (ATR) on myofibroblasts. (C) Ex vivo 2-photon microscopy demonstrated fluorescent APA intracellularly within the myofibroblast (green), with collagen surrounding the myofibroblast using second harmonic generation imaging (blue). Abbreviations as in Figure 1.