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Figure 4 Immunohistology Documenting Angiogenesis in VEGF-Injected Animals
Values from quantitative immunohistology in the ameroid territory for the vascular endothelial growth factor (VEGF)-injected animals (yellow bars) and saline-injected animals (orange bars) are shown in the upper panel. The VEGF-injected experiments showed less fibrosis and greater capillary sprouting by lectin staining than the saline-injected control experiments. The bottom images show representative examples of tissue staining. Panel A shows a myocardial section stained for lectin from the injection territory from 1 VEGF experiment, and panel B shows 1 saline-injected animal. Brown staining of sprouting capillaries is seen in panel A and is absent in panel B. Panel C shows green fluorescence identifying integrin expression in capillary sprouts from the injection site of 1 VEGF-injected animal that showed in-vivo uptake of [123I]Gluco-RGD. FITC = fluorescein.