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Figure 3 CPS Ultrasound Detects Blood Flow in Vessels With a Diameter of 10 to 20 µm
(A) Transversal CPS imaging at 8.0 MHz in AdVEGF-injected semimembranosus muscle 6 days after gene transfer. Note the dark area inside the muscle showing little CPS signal, owing to low basal perfusion typical for intact skeletal muscle (surrounded by a green line). (B) A histological section of the same location as in panel A, covering the whole muscle and immunostained with the CD31 endothelial marker. Normal-sized capillaries colocalize with very low CPS signal inside of the area marked by a green line, whereas enlarged capillaries outside of the area colocalize with a good signal in CPS. (C and D) Higher magnifications of the regions marked with black boxes in panel B demonstrate a clear demarcation between the areas with normal (black arrows) versus enlarged (diameter 10 to 20 µm, red arrows) capillaries. Asterisks denote enlarged veins, and arrowheads indicate the profound femoral artery in panels A to C. Scale bar = 200 µm. See Online Video 3. Abbreviations as in Figure 1.
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