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Figure 5 CPS Allows High-Resolution Perfusion Imaging in Mouse Hind Limbs After Angiogenic Gene Transfer
(A to D) Normal power Doppler without contrast enhancement (left) and 14 MHz CPS imaging (right) 6 days after AdLacZ or AdVEGF gene transfer in mouse hind limbs. (A and B) AdVEGF induces angiogenesis and perfusion increases almost exclusively in the subcutis (brackets), probably owing to the low tropism of adenovirus in mouse skeletal muscle. The femoral artery is indicated by arrowheads. (C and D) Also in the calf, angiogenesis mostly occurs in the adipose tissue (brackets). Here, arrowheads denote the popliteal artery. Lectin capillary stainings of skeletal muscle (E and F) and subcutis (G and H) nearby. Although there are a few slightly enlarged capillaries in skeletal muscle (F, arrows), angiogenesis mostly takes place in the adipose tissue. Note red blood cells inside enlarged blood vessels (asterisks), indicating that the vessels are perfused. Scale bar = 100 µm. See also Online Video 4. Abbreviations as in Figures 1 and 2.
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